This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. Thespecified metagenomics process includes the following stages:

a) sampling strategy and process, including type, storage, transportation, extraction, quality;

b) nucleic acid library preparation

c) design and review process including sequencing strategy and assessment;

d) database construction;

e) bioinformaticsanalysis and report

f) validation and verification for bioinformatics pipeline, and database

This document applies to laboratories and research organizations.

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